![]() ![]() Prenatal cannabis exposure (PCE) influences human brain development, but it is challenging to model PCE using animals and current cell culture techniques. ![]() ![]() (F) An additional population of Ki-67+ cells is found in an outer subventricular zone-like region (arrowheads). (E) Proliferating progenitor cells labeled by Ki-67 (green) localize along the ventricle, nuclei are counterstained with DAPI (blue). Organization of the layers recapitulates early corticogenesis observed during human brain development. (D) CTIP2, a marker of the developing cortical plate, co-localizes with β-tubulin III+ neurons in a cortical plate-like region. β-tubulin III+ neurons (green) are adjacent to the ventricular zone. (C) PAX6+ apical progenitors (red, enclosed by dotted line) are localized to a ventricular zone-like region. (B) Immunohistological analysis on cryosections of cerebral organoids reveals cortical regions within the organoid labelled by the apical progenitor marker PAX6 (red) and neuronal marker β-tubulin III (green). Cerebral organoids at this stage are made up of phase-dark structures that may be surrounded by regions of thinner, more translucent structures that display layering (arrowheads). ![]() (A) A representative phase-contrast image of a whole cerebral organoid at day 40 generated using the STEMdiff™ Cerebral Organoid Kit. Tissue and Cell Culture Dissociation Reagents.Work at STEMCELL View Current Opportunities > ![]()
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